Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/9503
Citations | ||
Scopus | Web of ScienceĀ® | Altmetric |
---|---|---|
?
|
?
|
Type: | Journal article |
Title: | A novel CD44 antibody identifies an epitope that is aberrantly expressed on acute lymphoblastic leukaemia cells |
Author: | Bendall, L. James, A. Zannettino, A. Simmons, P. Gottlieb, D. Bradstock, K. |
Citation: | Immunology and Cell Biology, 2003; 81(4):311-319 |
Publisher: | Blackwell Publishing Asia |
Issue Date: | 2003 |
ISSN: | 0818-9641 1440-1711 |
Statement of Responsibility: | Linda J Bendall ; Alexander James ; Andrew Zannettino ; Paul J Simmons ; David J Gottlieb ; Kenneth F Bradstock |
Abstract: | Previous studies have shown that the antibody 7H9D6 identifies CD44, a glycoprotein receptor for hyaluronic acid. 7H9D6 recognizes an epitope of CD44 that is not always present on CD44 molecules. The 7H9D6 antibody bound to the hyaluronic acid binding domain of CD44 and inhibited cell adhesion to immobilized hyaluronic acid. However, the expression of the 7H9D6 epitope was not sufficient for hyaluronic acid binding. Immunofluorescent staining with 7H9D6 revealed a punctate surface staining pattern, suggesting that CD44 molecules recognized by 7H9D6 are located in clusters on the cell surface. In contrast, other CD44 antibodies produced a uniform staining pattern. Early bone marrow B cells were negative for 7H9D6 but reactive with other CD44 monoclonal antibodies. In contrast, leukaemic cells from 65% of patients (28 of 43) with B lineage acute lymphoblastic leukaemia bound 7H9D6. Patients expressing the 7H9D6 epitope on their leukaemic cells had an increased risk of death (HR 3.5 95% CI 1.1-10.9, P = 0.029) and of disease relapse (HR 3.2 95% CI 1.2-8.5, P = 0.017) when corrected for white cell count. This antibody may be useful for the detection of residual disease in B lineage acute lymphoblastic leukaemia and as a prognostic indicator and for the study of CD44 function. |
Keywords: | B-Lymphocytes Bone Marrow Cells Cell Line Cell Line, Tumor Cell Membrane Humans Hyaluronic Acid Antigens, CD Antibodies, Monoclonal Epitopes Microscopy, Fluorescence Flow Cytometry Cell Adhesion Adolescent Middle Aged Child Child, Preschool Infant, Newborn Female Male Precursor Cell Lymphoblastic Leukemia-Lymphoma Hyaluronan Receptors |
DOI: | 10.1046/j.1440-1711.2003.t01-1-01174.x |
Published version: | http://dx.doi.org/10.1046/j.1440-1711.2003.t01-1-01174.x |
Appears in Collections: | Aurora harvest Medicine publications |
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.