Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/9723
Citations | ||
Scopus | Web of Science® | Altmetric |
---|---|---|
?
|
?
|
Type: | Journal article |
Title: | Dynamic methylation of histone H3 at lysine 4 in transcriptional regulation by the androgen receptor |
Author: | Kim, J. Jia, L. Tilley, W. Coetzee, G. |
Citation: | Nucleic Acids Research, 2003; 31(23):6741-6747 |
Publisher: | Oxford Univ Press |
Issue Date: | 2003 |
ISSN: | 0305-1048 1362-4962 |
Statement of Responsibility: | Joshua Kim, Li Jia, Wayne D. Tilley and Gerhard A. Coetzee |
Abstract: | The methylation of histone H3 correlates with either gene expression or silencing depending on the residues modified. Methylated lysine 4 (H3-K4) is associated with transcription at active gene loci. Furthermore, it was reported that trimethylated but not dimethylated H3-K4 is exclusively associated with active chromatin in Saccharomyces cerevisiae. In the present study, we investigated the H3-K4 methylation at the human prostate specific antigen (PSA) locus following gene activation and repression via androgen receptor (AR). We show that ligand-induced, AR-mediated transcription was accompanied by rapid decreases in di- and trimethylated H3-K4 at the PSA enhancer and promoter. Moreover, the observed decreases in H3-K4 methylation were reversed when AR was inhibited by a specific AR antagonist, bicalutamide. In contrast to the decreases in methylation at the 5' transcriptional control regions of the PSA gene, H3-K4 methylation in the coding region steadily increased after a lag period of 4 h. The results suggest a novel role of methylated H3-K4 in transcriptional regulation. |
Keywords: | Cell Line, Tumor Humans Anilides Tosyl Compounds Nitriles Prostate-Specific Antigen Lysine Histones Receptors, Androgen RNA, Messenger Transcription, Genetic Methylation Kinetics Exons Male Enhancer Elements, Genetic Promoter Regions, Genetic Transcriptional Activation Androgen Receptor Antagonists |
Description: | © 2003 Oxford University Press |
DOI: | 10.1093/nar/gkg909 |
Published version: | http://dx.doi.org/10.1093/nar/gkg909 |
Appears in Collections: | Aurora harvest Medicine publications |
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.