Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/99150
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Type: Journal article
Title: Multimerization of a proline-rich antimicrobial peptide, Chex-Arg20, alters its mechanism of interaction with the Escherichia coli membrane
Author: Li, W.
O'Brien-Simpson, N.
Tailhades, J.
Pantarat, N.
Dawson, R.
Otvos, L.
Reynolds, E.
Separovic, F.
Hossain, M.
Wade, J.
Citation: Chemistry and Biology, 2015; 22(9):1250-1258
Publisher: Elsevier (Cell Press)
Issue Date: 2015
ISSN: 1074-5521
1879-1301
Statement of
Responsibility: 
Wenyi Li, Neil M. O'Brien-Simpson, Julien Tailhades, Namfon Pantarat, Raymond M. Dawson, Laszlo Otvos, Eric C. Reynolds, Frances Separovic, Mohammed Akhter Hossain, John D. Wade
Abstract: A3-APO, a de novo designed branched dimeric proline-rich antimicrobial peptide (PrAMP), is highly effective against a variety of in vivo bacterial infections. We undertook a selective examination of the mechanism for the Gram-negative Escherichia coli bacterial membrane interaction of the monomer (Chex-Arg20), dimer (A3-APO), and tetramer (A3-APO disulfide-linked dimer). All three synthetic peptides were effective at killing E. coli. However, the tetramer was 30-fold more membrane disruptive than the dimer while the monomer showed no membrane activity. Using flow cytometry and high-resolution fluorescent microscopy, it was observed that dimerization and tetramerization of the Chex-Arg20 monomer led to an alteration in the mechanism of action from non-lytic/membrane hyperpolarization to membrane disruption/depolarization. Our findings show that the membrane interaction and permeability of Chex-Arg20 was altered by multimerization.
Keywords: Escherichia coli; Proline; Peptides; Antimicrobial Cationic Peptides; Membrane Proteins; Microscopy, Fluorescence; Flow Cytometry; Microbial Sensitivity Tests; Structure-Activity Relationship; Dimerization; Proline-Rich Protein Domains
Rights: © 2015 Elsevier Ltd. All rights reserved.
RMID: 0030048056
DOI: 10.1016/j.chembiol.2015.08.011
Grant ID: http://purl.org/au-research/grants/arc/DP150103522
http://purl.org/au-research/grants/nhmrc/1029878
http://purl.org/au-research/grants/nhmrc/1008106
Appears in Collections:Medicine publications

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