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https://hdl.handle.net/2440/74094
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Type: | Journal article |
Title: | Internal calibrants allow high accuracy peptide matching between MALDI imaging MS and LC-MS/MS |
Author: | Gustafsson, O. Eddes, J. Meding, S. Koudelka, T. Oehler, M. McColl, S. Hoffmann, P. |
Citation: | Journal of Proteomics, 2012; 75(16):5093-5105 |
Publisher: | Elsevier BV |
Issue Date: | 2012 |
ISSN: | 1874-3919 1876-7737 |
Statement of Responsibility: | Johan O.R. Gustafsson, James S. Eddes, Stephan Meding, Tomas Koudelka, Martin K. Oehler, Shaun R. McColl and Peter Hoffmann |
Abstract: | One of the important challenges for MALDI imaging mass spectrometry (MALDI-IMS) is the unambiguous identification of measured analytes. One way to do this is to match tryptic peptide MALDI-IMS m/z values with LC-MS/MS identified m/z values. Matching using current MALDI-TOF/TOF MS instruments is difficult due to the variability of in situ time-of-flight (TOF) m/z measurements. This variability is currently addressed using external calibration, which limits achievable mass accuracy for MALDI-IMS and makes it difficult to match these data to downstream LC-MS/MS results. To overcome this challenge, the work presented here details a method for internally calibrating data sets generated from tryptic peptide MALDI-IMS on formalin-fixed paraffin-embedded sections of ovarian cancer. By calibrating all spectra to internal peak features the m/z error for matches made between MALDI-IMS m/z values and LC-MS/MS identified peptide m/z values was significantly reduced. This improvement was confirmed by follow up matching of LC-MS/MS spectra to in situ MS/MS spectra from the same m/z peak features. The sum of the data presented here indicates that internal calibrants should be a standard component of tryptic peptide MALDI-IMS experiments. |
Keywords: | Humans Carcinoma Ovarian Neoplasms Peptides Observer Variation Diagnostic Imaging Calibration Chromatography, Liquid Peptide Mapping Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Microtomy Reproducibility of Results Sequence Analysis, Protein Amino Acid Sequence Female Tandem Mass Spectrometry |
Rights: | © 2012 Elsevier B.V. All rights reserved. |
DOI: | 10.1016/j.jprot.2012.04.054 |
Grant ID: | http://purl.org/au-research/grants/arc/LP110100693 http://purl.org/au-research/grants/arc/LP110100693 |
Published version: | http://dx.doi.org/10.1016/j.jprot.2012.04.054 |
Appears in Collections: | Aurora harvest 4 IPAS publications Molecular and Biomedical Science publications |
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